Hassan Akrami

     Assistant Professor in Molecular Genetics

      Address:
Department of Biology, School of Science, Razi University, Kermanshah, Iran
Phone+98(831)4274545,     
Fax: +98(831)4274555
Zipcode:   6714414676
                   h.akrami@razi.ac.ir
Education:

Year
Degree
Major
University
2004- 2010
PhD
Molecular Genetics
TMU, Iran
1996 -1998
MSc
Genetics
TMU, Iran
1993 - 1996
BSc
 Animal Biology
Razi University, Iran
1988- 1992
Diploma
Science
Shiraz, Iran
Position:

2010- Present: Assistant Professor in Molecular Genetics, Department of Biology, School of Science, Razi University, Kermanshah, Iran.
Technical Skills and experience:

Molecular and cell biology techniques including:
 Gene cloning techniques (Vector design, Manipulation and transformation)
 Cell culture techniques
 Tissue engineering
 RNAi technology
 PCR techniques (Primer design, Quantitative Real-time PCR, PCR-Microarray, RAPD, RT-PCR)
 Blotting (Southern, Northern and western blotting)
 Polyacrylamide gel electrophoresis (PAGE, SDS-PAGE, Zymography)
Thesis research:
Ph.D. Dissertation:
Evaluation of the PlGF gene expression knocking down in Human retinal pigment epithelium (RPE) cells to block Choroidal neovascularization (CNV). Supervisor: Dr. Z. S. Soheili
      M.Sc. Thesis:
Cloning and Expression of the Human Interferon α2b in E.coli. Supervisor: Dr. F. Mehraein
Work and teaching experience:
         
        
Teaching experiences
 1999 – Present, teaching undergraduate courses: Basic Genetics, Molecular Genetics, Human Genetics, Cell Biology.
 2010 – Present, teaching Postgraduate courses: Genetic Engineering, Molecular Biotechnology, Genetic of Prokaryotes, Molecular Oncology.
         Work Experiences
2001- 2004: NIGEB, P.O.Box: 14155-6343 Tehran-Iran
1998-2001: Pasteur Institute, Tehran-Iran
Research Interests:

 Gastric cancer signalling pathways
 Cancer stem cell
 Cancer Therapy
Publications and presentations (In reverse chronological order):
Books
1.      H. Akrami. (2009) Genetics (Classic to Genomic). House of Biology, (In Persian)
Papers
1.      F. Mahmoodi, H. Akrami*. Evaluate the inhibitory effect of ibuprofen on metastasis and invasion in Gastric cancer stem cells. J Shahrekord Univ Med Sci.2015; 17(4): 88-96. (Scientific- Research,ISC; In Persian)
2.      S. Mirzaaghaei, H. Akrami*, M. H. Asadi, K. Mansouri, H. Mahdiuni. Ferula gummosa Boiss flower and leaf extracts inhibit angiogenesis in vitro. Indian J of Cancer(Accepted, 2013; ISI, IF: 1.131)
3.      H. Akrami*, S. Aminzadeh, H. Fallahi. Inhibitory effect of ibuprofen on tumor survival and angiogenesis in gastric cancer cell. Tumor Biology.2015; 36(5): 3237–3243. (ISI, IF: 3.61)
4.      H. Akrami, M. Aminzadeh, A. Sharifi*. The Effect of Plgf Gene Knockdown on Enzymes Metalloproteinase-2 and -9 (MMP-2&-9) Activity. Medical J Tabriz Uni MedSci Health Services. 2014; 36(4): 10-13. (Scientific- Research, ISC; In Persian)
5.      Z. Ghambarali, A. Bidmeshkipure*, H. akrami, M. Azadbakht and A. Rabzia. Ethanolic extract of Ficus carica leave Suppresses Angiogenesis by Regulating VEGF-A and Integrin β3mRNA Expression in Human umbilical vein endothelial cells. Indian J Physiol Pharmacol. 2014; 58(4): 407–415. (Non ISI)
6.      S. Heidari, H. Akrami*, R. Gharaei, A. Jalili, H. Mahdiuni, E. Golezar. Anti-tumor activity of Ferulago angulata Boiss. extract in gastric cancer cell line via induction of apoptosis. Iran J Pharm Res.2014; 13 (4): 1335-1345. (ISI, IF: 0.51)
7.      S. MirzaAghaei, H. Akrami*, K. Mansouri. Ferulago angulata flower and leaf extracts inhibit angiogenesis in vitro via reducing the VEGF-A and VEGFR-2 genes expression. Arch Iran Med. 2014; 17 (4): 278-285. (ISI, IF: 1.22)
8.      A. Sharifi, M. Aminzadeh-Bukani,H. Akrami*, M. Rahbani Nobar, S. Havasi. Metastasis Inhibition through Down Regulation of PlGF in Gastric Adenocarcinoma (AGS) Cells. Medical J Tabriz Uni MedSci Health Services. 2013; 35(4): 52-57. (Scientific- Research, ISC; In Persian)
9.      H. Akrami, A. Hedayati, M. Farshian, G. Haqshenas*. Development of a Recombinant Protein-based Dot-blot Hybridization Assay for the Detection of Antibody to Chicken Infectious Bronchitis Virus. Iran J Vet Res. 2013; 14 (4): 350-353 (ISI, IF: 0.159)
10. M. Davari, z. soheili*, H. Ahmadieh*, F. Sanie-Jahromi, S. Ghaderi, M. Rezaie Kanavi, S. Samie, H. Akrami, M. Haghighi, and F. Javidi-Azad. Amniotic Fluid Promotes the Appearance of Neural Retinal Progenitors and Neurons in Human Retinal Pigment Epithelial cell Cultures. Molecular Vision. 2013; 19: 2330-2342 (ISI, IF: 2.4)
11. R. Nourinia, Z. S. Soheili*, H. Ahmadieh, H. Akrami, M. Rezaei Kanavi, S. Samiei. Knockdown of the Placental Growth Factor Gene Inhibits Laser Induced Choroidal Neovascularization in a Murine Model. J Ophthalmic Vis Res. 2013; 8 (1): 4-8.(ISC)
12. R. Gharaei, H. Akrami*, S. Heidari, M. H. Asadi, A. Jalili. The suppression effect of Ferula gummosa Boiss extracts on cell proliferation through apoptosis induction in gastric cancer cell line. Eur J Integr Med. 2013; 5 (3): 241–247. (ISI, IF:0.649)
13. H. Akrami,Z. S. Soheili*,M. Sadeghizadeh,H. Ahmadieh, M. Rezaie Kanavi, S. Samiei, K. Khalooghi. PlGF Gene Knockdown in Human Retinal Pigment Epithelial Cells. Graefes Arch Clin Exp Ophthalmol. 2011; 249 (4): 537-546.(ISI, IF: 2.17)
14. H. Akrami, Z. S. Soheili*, M. Sadeghizadeh, K. Khalooghi, H. Ahmadieh, M. Rezaie Kanavi, S. Samiei, J. Pakravesh. Evaluation of RPE65, CRALBP, VEGF, CD68 and tyrosinase gene expression in Human Retinal Pigment Epithelial cells cultured on amniotic membrane.Biochem Genet. 2011; 49 (5): 313-322. (ISI, IF: 0.87)
15. H. Akrami, Z. S. Soheili*, K. Khalooghi, H. Ahmadieh, M. Rezaie-Kanavi, S. Samiei, M. Davari, S. Ghaderi, F. Sanie-Jahromi. Retinal Pigment Epithelial Cell Culture; a Potential Source for Retinal Stem Cells. J Ophthalmic Vis Res. 2009; 4 (3): 1-10. (Scientific- Research)
16. G. Haqshenas*, K. Assasi and H. Akrami. Isolation and Molecular Characterization of infectious Bronchitis Virus, IsolateShiraz3 IBV, By RT-PCR and Restriction Enzyme analysis. Iran J Vet Res. 2005; 6 (2):9-15.(Scientific- Research)
17. G. Haqshenas*, H. Akrami, and M. Shayegh. Molecular Cloning and Expression of Nucleocapsid gene of Chicken Infectious Bronchitis Virus strain Massachusetts H120. J. Sci. I. Iran. 2004; 15 (3): 211- 218.(Scientific- Research)
18. G. Ahangary*, H. Akrami, M. H. Sanati, A. Rabbani. Antibody Production against ECP by Dot blotting and ELISA for evaluating ECP in recombinant drugs and patients serum. I. J. Pediatrics. 2003; 13 (1): 3-8.(Scientific- Research, In Persian)
19. F. Mehraein*, H. Akrami, M. R. Noori-Daloii, A. Mostafaii and M. Sadeghizadeh. Cloning and Expression of the Human Interferon α2 in E.coli. J. Sci. I. Iran. 1999; 10 (2): 85-92. (Scientific- Research)
Abstracts
1.      R. Tayyebi, A. Bidmeshkipour, H. Akrami, Z. Ghambarali. Anti-proliferative effect of ethanolic extract of fig (Ficoscarica) leaf on human gastric cancer AGS cell line. The 17 National & 5th International Iranian Biology Conference. Kerman, Iran. 4th to 6th September 2012.
2.      S. Mirzaaghaei, H. Akrami, K. Mansouri. Ferula gummosa Boiss leaves can inhibit HUVECs proliferation. The 17 National & 5th International Iranian Biology Conference. Kerman, Iran. 4th to 6th September 2012.
3.      S. Mirzaaghaei, H. Akrami, K. Mansouri, H. Fallahi. Anti-angiogenic activity of ethanol extracts of Ferulago Angulata leaves. 12th Iranian Congress of Biochemistry & 4th International Congress of Biochemistry and Molecular Biology, Mashhad, Iran. September 6-9 Clinical Biochemistry Vol.44 No.13S Sep, 2011.
4.      R. Gharaei, S. Ghobadi, H. Akrami. Study of apoptosis inducing effects of ethanol extract of Ferulla gummosa leaf. 12th Iranian Congress of Biochemistry & 4th International Congress of Biochemistry and Molecular Biology, Mashhad, Irano September 6-9 Clinical Biochemistry Vol.44 No.13S Sep, 2011.
5.      S. Heidari, H. Akrami, H. Mahdiuni. Study of Chemopreventive activity of ethanolic extract of Ferulago angulata leaves. 12th Iranian Congress of Biochemistry & 4th International Congress of Biochemistry and Molecular Biology, Mashhad, Irano September 6-9 Clinical Biochemistry Vol.44 No.13S Sep, 2011.
6.      Z. Ghambarali, M. Azadbakht, A. Bidmeshkipour, H. Akrami, A. Rabzia. Anti-migratory effect of ethanolic extract of fig (Ficus carica) leaf on human umbilical vein endothelial cells. 12th Iranian Congress of Biochemistry & 4th International Congress of Biochemistry and Molecular Biology, Mashhad, Irano September 6-9 Clinical Biochemistry Vol.44 No.13S Sep, 2011.
7.      A. Rabzia, M. Azadbakht, A. Bidmeshkipour, H. Akrami. The effect of different concentrations of morphine and extracellular Ca2+ on cell death in PC12 cell line. 12th Iranian Congress of Biochemistry & 4th International Congress of Biochemistry and Molecular Biology, Mashhad, Irano September 6-9 , Clinical Biochemistry Vol.44 No.13S Sep, 2011.
8.      M. Davari, H. Ahmadieh, Z. S. Soheili, F. Sanie-Jahromi, S. Ghaderi, M. Rezaie, S.Samie, H. Akrami, J. Pakravesh. Manifestation of Retinal Neurons and Progenitors in Retinal Pigment Epithelium Cultures Treated by Amniotic Fluid. ISER, the XIX Biennial Meeting of the International Society for Eye Research, Canada, 18- 23 July, 2010.
9.      S. Samiei, H. Akrami, H. Ahmadieh, M. Rezaie Kanavi, Z.S. Soheili, M.Sadeghizadeh. Down Regulation of PlGF Reduced angiogenesis potential in Retinal Pigment Epithelial Cell conditioned media. ARVO International society for Ocular Cell Biology (ISOCB), Ericeira Portugal, 9-12 Sep, 2009.
10. Z. S. Soheili, H. Akrami, K. Khalooghi, H. Ahmadieh, M. Rezaie Kanavi, S. Samiei . Generation of Retinal progenitor (stem) cells from human cultured RPE cells. Euretina Congress 22-25 May, 2008. 
11. Z. S. Soheili, H. Akrami, K. Khalooghi, H. Ahmadieh, M. Rezaie Kanavi, S. Samiei . Retinal pigment epithelial cell culture on Amniotic Membrane. Euretina Congress 22-25 May, 2008.  
12. Z. S. Soheili, H. Akrami, K. Khalooghi, H. Ahmadieh, M. Rezaie Kanavi, S. Samiei . Generation of Retinal progenitor (stem) cells from human cultured RPE cells. The 5th National Biotechnology Congress of Iran, 24-26 Nov, 2007.
13. K. Khalooghi, H. Akrami, H. Ahmadieh, M. Rezaie Kanavi, Z. S. Soheili. Evaluation of different RPE cell isolation methods from human eye globes. The 9th Iranian Congress of Biochemistry & the 2nd International of Biochemistry and Molecular Biology, Shiraz-Iran, 29 Oct - 1 Nov, 2007.
14. Ahangari G, Goodarzi A, H. Akrami, Mirsepasi G, Zarindast MR.RT-PCRBased Analysis of Dopamine Receptor Gene Rearrangement in Peripheral BloodLymphocytes in Schizophrenia Patients. The third European-American school in forensic genetics and mayo clinic coarse in advanced molecular and cellular medicine in Zegreb-Croatia, 2003.
15. F. Mehraein, H. Mirzahoseini, H. Akrami. Developing a Versatile Vectorfor High Level Expression of an Interferon a2 cDNA in E.coli. The 8th Iranian Genetics Congress, 2003.
16. F. Mehraein, R.Fooladi, K.Pooshang Bagheri, H. Akrami and S. Altaie.Prevalence of Methicillin Resistant Staphylococcus aureus (MRSA) that are alsoGlycopeptide/Vancomycin Intermediate (GISA/VISA) in Tehran, Iran. General Meeting ASM Abstract data base, 2001.
17. H. Akrami, F. Mehraein, A. Nazemi. Study of a Interferon α2 protein inE.coli Periplasmic Space. Ninth Iranian Biology Conferance, Faculty of science, University of Tehran, 2000.
18. H. Akrami, F. Mehraein, M.R. Noori-Daloii, and M.Sadeghizadeh. Cloning and Expression of the Human Interferon α2 in E.coli. 14th Iranian Congress of Physiology and Pharmacology. Tehran University of Medical Science, 16-20 May, 1999.
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